https://repositorio.ufpb.br/jspui/handle/123456789/2374 Sat, 13 Jun 2026 23:37:58 GMT 2026-06-13T23:37:58Z https://repositorio.ufpb.br/jspui/handle/123456789/38162 Título: Mecanismos de regulação cardiometabólica induzidos pelo exercício físico na prevenção da aterosclerose: uma análise in silico Autor(es): Silva, Daniele Diniz da Orientador: Martin, Christina Pacheco Santos Abstract: Atherosclerosis is a chronic, multifactorial inflammatory pathology and a leading cause of global mortality, necessitating the investigation of robust preventive strategies. This study aimed to investigate, through bioinformatics and systems biology approaches, the molecular mechanisms by which physical exercise modulates cardiometabolic pathways and prevents disease progression. The methodology consisted of quantitative and descriptive in silico research, based on cross-referencing transcriptomic data from the Fitnoma catalog with the atherosclerosis pathway from the KEGG database, followed by the construction of Protein-Protein Interaction (PPI) networks via the STRING platform and functional enrichment analysis using WebGestalt. The results identified 42 common genes, revealing a molecular network with remarkably high connectivity. The PLCG1 gene emerged as the primary hub involved in calcium signaling and eNOS enzyme activation—processes essential for maintaining vascular homeostasis. Furthermore, critical axes for cell survival (PI3K-AKT) and inflammatory modulation (TLR4-MAPK) were identified. Enrichment analysis highlighted immune system regulation as the central biological process mediated by exercise. In conclusion, physical activity exerts a systemic cardioprotective impact by acting as a coordinated biological cluster that mitigates systemic inflammation, reverses cellular damage, and stabilizes atherosclerotic plaques through gene transcription modulation. These findings validate the use of computational tools for discovering therapeutic Editor: Universidade Federal da Paraíba Tipo: TCC Fri, 27 Mar 2026 00:00:00 GMT https://repositorio.ufpb.br/jspui/handle/123456789/38162 2026-03-27T00:00:00Z https://repositorio.ufpb.br/jspui/handle/123456789/38161 Título: Análise in-silico da regulação gênica pelo exercício físico sobre as vias moleculares ligadas à doença de Parkinson Autor(es): Silva, Gabryella Hellen Maracajá Coutinho da Orientador: Martin, Christina Pacheco Santos Martin Abstract: Physical exercise has been an important non-pharmacological treatment for chronic non-communicable diseases, such as Parkinson's Disease, a progressive neurodegenerative disease that primarily affects the central nervous system and whose incidence is increasing. Therefore, the objective of this work is to analyze gene regulation of molecular pathways linked to Parkinson's Disease through physical exercise, aiming to provide evidence supporting the practice of exercise as a non-pharmacological treatment and to generate subsidies for the development of biomarkers. The methodology employed was based on the FITNOME Catalog and through an analysis of bioinformatics platforms such as SportsXbiodata, WebGestalt, KEGG, STRING, miRWalk, and Cytoscape for filtering and integration, enrichment, mapping, protein interactions, microRNA interactions, and network visualizations of the interactions. Thus, the results of this work demonstrate the regulation of all genes associated with this disease through physical exercise, indicating an adaptive modulation of these genes induced by physical exercise. This modulation results in the regulation of biological processes mainly related to mitochondrial autophagy, in addition to promoting metabolic and molecular adjustments in cells. Interactions between proteins were shown to be highly branched, revealing a large cascade effect. Furthermore, interactions between microRNAs and genes highlighted central hubs as potential therapeutic targets and biomarkers. Finally, in conclusion, physical exercise appears to be a promising non-pharmacological treatment for Parkinson's Disease, as shown by all the analysis performed; physical exercise acts as an adaptive regulator of Parkinson's genes. Thus, this work clarifies and opens new avenues for research on the regulation of Parkinson's Disease through physical exercise, indicating a relevant mechanism with possible overall metabolic improvement of the genes and proteins involved in the regulatory processes of this disease. Editor: Universidade Federal da Paraíba Tipo: TCC Mon, 30 Mar 2026 00:00:00 GMT https://repositorio.ufpb.br/jspui/handle/123456789/38161 2026-03-30T00:00:00Z https://repositorio.ufpb.br/jspui/handle/123456789/38157 Título: Terapia CAR-T: avanços e perspectivas no Brasil Autor(es): Souza, Maria Beatriz Nascimento Marinho de Orientador: Dejani, Naiara Naiana Abstract: CAR-T cell therapy (Chimeric Antigen Receptor T-cell therapy) represents one of the most advanced immunotherapy strategies in the treatment of hematological malignancies. This study aimed to list the drugs approved by ANVISA (Brazilian Health Regulatory Agency) for CAR-T therapy in Brazil, discuss their main therapeutic targets, analyze the progress and future prospects of the therapy in the country, identify its main obstacles, and clarify the eligibility criteria for patients and access to treatment. This is an integrative narrative review, conducted in the PubMed, SciELO Brazil, LILACS, and CAPES Periodicals databases, supplemented by institutional documents, regulatory reports, and official clinical trial records. The systematic search did not identify original articles with complete clinical results published in Brazil, due to the fact that the first national clinical trial, the CARTHEDRALL Study (NCT06101381), is still ongoing. The materials found include reviews, institutional communications, and technical updates from organizations such as ANVISA, INCA, Instituto Butantan, Fundação Hemocentro de Ribeirão Preto, and FIOCRUZ. The results indicate that Brazil is advancing in the production and clinical application of the therapy, with national experimental platforms and a growing laboratory infrastructure. However, challenges persist, such as the absence of fully consolidated regulatory frameworks, high costs, production complexity, and limited patient access. It is concluded that the country possesses increasing scientific and technological capacity to consolidate CAR-T therapy, provided there is a strengthening of public policies, expansion of research, and integration between health centers, universities, and regulatory bodies, enabling this innovative technology to become accessible and sustainable within the Unified Health System (SUS). Editor: Universidade Federal da Paraíba Tipo: TCC Wed, 01 Apr 2026 00:00:00 GMT https://repositorio.ufpb.br/jspui/handle/123456789/38157 2026-04-01T00:00:00Z https://repositorio.ufpb.br/jspui/handle/123456789/38156 Título: Produção de nanocápsulas poliméricas de óleo de copaíba: aspectos físico-químicos e atividade antimicrobiana Autor(es): Silva, Vinicius Bispo da Orientador: Xavier Júnior, Francisco Humberto Abstract: Skin infections are a common condition in humanity and can evolve into resistance if not treated correctly. The metabolites present in copaiba essential (CEO) and fixed (CFO) oils are an alternative, since they have already been shown to inhibit microorganisms. However, their therapeutic use, especially if associated with delivery nanosystems, enhances their action, favoring passage through the defense mechanisms of microorganisms. Therefore, this study aimed to evaluate the physicochemical and antimicrobial aspects of polymeric nanocapsules (PNCs) carrying copaiba fixed and essential oils. To this end, the oils were characterized by gas chromatography coupled to mass spectrometry, and their antioxidant action was evaluated by DPPH and ABTS radical reduction methods. The PNCs were developed, varying the polymers polycaprolactone 10 kDa (PCL-10) and 80 kDa (PCL-80) (25─200 mg), the oils (CEO, CFO, 15─200 mg) and the surfactants Span®60, Lipoid®s100, Tween®20, Tween®80 and Kolliphor®ELP (20─100 mg). The PNCs were characterized by mean size, polydispersity index (PdI), Zeta potential, infrared spectroscopy (FTIR), optical microscopy and scanning electron microscopy (SEM), drug loading and encapsulation efficiency (EE%). Complexation with collagen and stability at 4 and 25 °C for 90 days of the final CEO (NcOEsC) and CFO (NcOFiC) nanocapsules were analyzed. Furthermore, the antimicrobial and antibiofilm activities of the oils, NcOEsC and NcOFiC were evaluated against Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 13150) and Candida albicans (ATCC 76645). CEO had β-caryophyllene (44.30%) as its major compound, while CFO had a chromatographic profile indicative of the presence of diterpenes. CEO showed antioxidant activity in DPPH, with an EC50 of 45.76 mg/mL; CFO reduced 9.44% regardless of the concentration used. CEO showed no activity in the ABTS test. PCL-10 and PCL-80 had saturation at 150 and 40 mg in the nanosystems, respectively, with higher amounts negatively influencing mean size and PdI. The stable oil limit concentration was 100 mg, with higher concentrations negatively interfering with mean size and PdI and leading to oil externalization, as observed by optical microscopy and infrared transmittances. The studied PNCs showed complex behavior in surfactant variation, which led to different results depending on the concentrations and types of surfactants used. The size, PdI and Zeta potential of NcOEsC were 216.03±1.63 nm, 0.22±0.01 and -29.65±0.68 mV, while NcOFiC were 243.37±2.89 nm, 0.14±0.05 and -24.26±0.44 mV. These nanocapsules had FTIR spectra similar to PCL-80 and Kolliphor®ELP and spherical shapes in SEM. NcOEsC showed a drug loading of 20.02±0.25 mg/ml and an EE% of 99.99%, and it was not possible to perform these tests with NcOFiC. They had low interaction with incubations from 1 to 40 mg collagen/ml and low interaction with incubation of 5 mg collagen/ml over 24 hours. PNCs were stable at the observed temperatures of 4 and 25°C, except for NcOFiC which destabilized within 60 days at 25°C. CEO showed a Minimum Inhibitory Concentration (MIC) of 1024 μg/ml against the studied microorganisms, but no antibiofilm activity. CFO showed no biological activity. NcOEsC showed no activity against C. albicans, but had an MIC against P. aeruginosa, E. coli and S. aureus of 0.5, 128 and 128 μg/ml and a biofilm inhibitory activity at 64, 128 and 128 μg/ml, respectively. NcOFiC had an MIC against P. aeruginosa, E. coli, S. aureus and C. albicans of 0.5, 16, 32 and 16 μg/ml and a biofilm inhibitory activity at 16, 16, 32 and 16 μg/ml, respectively. In conclusion, PNCs helped improve the performance of copaiba oils against skin pathogens. Editor: Universidade Federal da Paraíba Tipo: TCC Tue, 03 Mar 2026 00:00:00 GMT https://repositorio.ufpb.br/jspui/handle/123456789/38156 2026-03-03T00:00:00Z