Cinética da produção de amilases produzido por Aspergillus sp. FSDE16 em bagaço de malte

Abstract

Brazil has a large quantity of malt bagasse available in the market with low cost, malt bagasse can be used as support and source of nutrients for microorganisms that produce enzymes in solid state cultivation processes. Enzymes are organic components capable of catalyzing chemical reactions. Enzymatic technology is today one of the most promising fields within the new technologies for the synthesis of high added value compounds, when compared to chemical catalysts they have an important characteristic that is the substrate specificity and to promote only a biochemical reaction with its substrate. Species of the genus Aspergillus have been used for the production of enzymes. Therefore, this work carried out the cultivation of Aspergillus sp. FSDE16 in 70% moisture malt bagasse using two types of humidification solution: (3.3 g/L ammonium sulfate, 1.5 g/L potassium phosphate) and water only. The best activity results were obtained with humidification solution with salts, the 120h culture with CMCase values 13.786 U/g, FPase 0.761 U/g and Amylase 18.336 U/g. The best pH range for amylase activity was between 4.5 and 5 and the best temperature for amylase activity was 70 °C. In this way the fungus Aspergillus sp. FSDE16 presented malt bag enzyme production capacity.