Desenvolvimento e caracterização de esponjas de quitosana e nanopartículas de policaprolactona carreando ácido tranexâmico

Abstract

This study aimed at to produce and to characterize of chitosan sponges (CS) and 2 polycaprolactone (PCL) nanoparticles loading tranexamic acid (ATX). The nanoparticles 3 were synthesized by solvent emulsification/evaporation technique and analyzed through 4 profilometric characterization, zeta potential (ZP), particle size (Z-Ave) and polydispersity 5 index (PdI). The inferential analysis was carried out through t-student test (α = 5%). The 6 respective values (mean ± standard deviation) of height by the profilometry, ZP, Z-Ave and 7 PdI of the NanoPCL were: 361 ± 82.93, -34.77 ± 1.317, 216 ± 2.018 and 0.1147 ± 0.005044; 8 for the NanoPCL/ATX: 115.1 ± 21.04, -27.73 ± 0.6173, 154.4 ± 0.6351 and 0.1377 ± 9 0.02018. The sponges were obtained by the lyophilization technique and characterized by 10 profilometry. It was observed that the lack of chitosan leaded to no sponge formation. The 11 statistical analysis of surface roughness (Sa) and mean roughness (Ra) was performed using 12 the two-way ANOVA test (α = 5%). There were differences between the mean roughness 13 values of the 75CS/25PCL-ATX, 75CS/25PCL, 50CS/50PCL-ATX and 50CS/50PCL 14 sponges (p<0,05). This study synthesized and characterized satisfactorily CS/PCL-ATX 15 sponges with possible therapeutic potential for hemostatic management.