Estudo in situ, cego, pareado, randomizado do cinamaldeído na desinfecção de próteses removíveis

Abstract

Objective: This blind, paired, randomized in situ clinical study investigated the effect of cinnamaldehyde on complete denture disinfection. Method: Test specimens (disks) were inserted into the prostheses of 33 users that as a probabilistic sample used solutions of cinnamaldehyde (200 μg/mL) and sodium hypochlorite (0.5%) in a paired and crossed manner for disinfection of their dentures. To determine the concentration of cinnamaldehyde to be used in the in situ study, its effect was first evaluated in vitro, on Candida spp biofilm. The disks were analyzed for the presence of microorganisms before and after the use of the products, on the 7th and 14th days, respectively; counting colony forming units (CFU) and scanning with an electron microscopy (SEM). The properties of the acrylic resin (roughness and Vickers hardness) were measured at the beginning and after immersion, with color parameters analyzed using the National Bureau of Standards (NBS) method. The data were analyzed by statistical tests, considering α = 5%. Results: The cinnamaldehyde concentration capable of significantly reducing Candida spp. biofilm was 195 μg/mL (p <0.05). A significant reduction (p <0.05) in the number of CFU/mL between the 7th and 14th day of use was observed for each type of microorganism (total microorganisms, from the mutans group and Candida spp.), with no significant differences (p> 0.05) between hypochlorite and cinnamaldehyde. There was an increase in the roughness and a decrease in the hardness of the test specimens, again with no difference between the two disinfectant substances (p> 0.05). Both hypochlorite and cinnamaldehyde also caused changes in color considered perceptible by NBS classification, but without significant difference between products (p <0.05). Conclusion: Similar to sodium hypochlorite, the solution containing cinnamaldehyde at 200 μg/mL presented efficacy against all evaluated microorganisms, caused changes in Vickers hardness, surface roughness and color parameters without clinical relevance, and can be associated with a mechanical method for the cleansing and disinfection of dentures and the prevention of prosthetic stomatitis.