Avaliação da atividade antiulcerogênica, antiinflamatória intestinal e antidiarreica da espécie Hyptis suaveolens (L.) Poit. (Lamiaceae) em modelos animais.

Abstract

Hyptis suaveolens L. Poit. (Lamiaceae) is used in the treatment of gastric problems and inflammation. This study aimed to evaluate the duodenal anti-ulcer, intestinal antiinflammatory and anti-diarrheal activity of the crude ethanol extract (EtOHE-Hs) and the hexane phase (HexFa-Hs) obtained from aerial parts of Hyptis suaveolens. In the duodenal ulcer model induced by cysteamine, the EtOHE-Hs (31.25, 62.5, 125 mg/kg) and lansoprazole (30 mg/kg) reduced the ALU in 47, 61, 42 and 59% (p<0,001), respectively, while HexFa-Hs (31.25, 62.5, 125 and 250 mg/kg) and lansoprazole (30 mg/kg) reduced this parameter in 79, 84, 89, 72 and 58%, when compared to the respective negative control group. In the model of acute ulcerative colitis induced by TNBS the EtOHE-Hs (31.25, 62.5, 125 mg/kg) and prednisolone (2 mg/kg) significantly reduced the in ALU 63 (p<0,001), 76 (p<0,001), 32 (p<0,05) and 74% (p<0,001), and the lesion score to 5 (4-8); p<0.05, 4 (3-5); p<0.01 and 5 (2-8); p<0.05, both compared to their respective colitics groups. Besides, the HexFa-Hs (62.5, 125 mg/kg) and prednisolone (2 mg/kg) reduced the ALU in 50, 55 and 56% (both p<0.001 and the lesion score 5 (4-7), 5 (4-5) and 5 (4-6) (both p<0.01) when compared to their respective colitics groups. In the chronic model of ulcerative colitis induced by TNBS, the EtOHE-Hs (62.5 mg/kg) did not promote this reduction in parameters, already HexFa-Hs (125 mg / kg) and prednisolone (2mg / kg) reduced the damage score for 2 (1-3); p <0.01 and 1 (1-4); p <0.001, the weight / length 139 ± 26 (p <0.001) and 139 ± 80 (p <0.001) and diarrhea to 61% (p <0.001) and 47% (p<0.001), respectively, compared to colitic group. In the acute model of ulcerative colitis the EtOHE-Hs (31.25, 62.5 mg/kg) and HexFa-Hs (125 mg / kg) increase the levels of reduced glutathione (GSH) compared to colítico group p <0.01, p <0.05 and p <0.001, respectively. In the chronic model of colitis the HexFa-Hs (125 mg / kg) significantly increased the GSH levels (p <0.001), catalase (p <0.001) and superoxide dismutase (p<0.01) reduced the levels of malondialdehyde (p <0.001) and nitrite / nitrate (p <0.05) and myeloperoxidase (p <0.001) compared to the respective values of the colitic group. The HexFa-Hs (125 mg / kg) reduce interleukin-1 beta levels (IL-1β) and tumor necrosis factor alpha (TNF-α), both with p <0.001 and increased levels of interleukin 10 (IL-10) p <0.001 compared to colíticos groups. In the model of diarrhea induced by castor oil, EtOHE-Hs (500 mg/kg), the HexFa-Hs (500 mg/kg) and loperamide (5 mg/kg) significantly reduced the evacuation index (EI) when compared to the negative control with p <0.01, p<0.01 and p <0.001, respectively. The HexFa-Hs (500 mg / kg) and loperamide significantly reduced gastric emptying and intestinal transit to 69% (p <0.001) and 47% (p <0.001), respectively, and 35% (p <0.001) and 21% (p <0.001) compared to the respective negative control. The antimotility activity FaHex-H involves nitric oxide share, KATP, cholinergic and adrenergic.