Diversidade genética em população de Portulaca umbraticola acessada por marcador RAPD

Abstract

The species Portulaca umbraticola has its use as ornamental, mainly in houses and gardens, because it has flowers with great phenotypic diversity among its individuals, these variations are entirely related to its genotype, therefore it is of utmost importance to evaluate the genetic diversity of the species to perform crosses and obtain new cultivars. The objective of this work was to analyze the genetic diversity of P. umbraticola using RAPD marker. The study was carried out in the plant biotechnology laboratory of the Agricultural Sciences Center of the Federal University of Paraíba, and used 18 accesses of P. umbraticola from the city of Areia/PB and João Pessoa/PB. For the extraction of the DNA was used tissue from the root of each individual, following the protocol of Doyle and Doyle, for the PCR reactions a total of 14 RAPD primers were used. The discrete DNA bands generated were responsible for the creation of a binary matrix, from which the dissimilarity matrix was constructed using the binary genetic distance of Sokal, and the clustering using the method of Ward.d2 by dendrogram. All amplified bands indicated polymorphism between the 18 accessions, with a total of 100% polymorphism, the highest distance between the accessions was 0.7661 between PU-12 and PU-16, through the dendrogram it was possible to form 3 distinct groups, already in the cluster of Tocher 6 groups were formed. Considering the matrix of dissimilarity, it is suggested crosses between accesses 12 and 16 because they have the greatest genetic distance between the accessions.