Atividade antifúngica do Cinamaldeído, a-Terpionel e própolis vermelha frente à biofilmes de Candida spp.

Abstract

Candida spp infections are prevalent in the oral cavity, being indicated the use of drugs such as nystatin, fluconazole, miconazole. In specific infections such as peri-implantitis, the use of mouthwashes with chlorhexidine is recommended. However, adverse effects are observed and, therefore, recent studies seek therapeutic alternatives based on products of natural origin. The objective of this study was to evaluate the inhibitory effect of cinnamaldehyde, -terpineol and red propolis on biofilms of Candida spp. In the first study, the Minimal Inhibitory Concentration (MIC) of the -terpineol and cinnamaldehyde were evaluated, using the serial microdilution technique against standard strains of C. albicans (ATCC 90028), C. glabrata (2001) and C. krusei (ATCC 34135), in addition to clinical isolates of C. albicans. The MIC for α-terpenol was found to range from 312.5 μg / ml to 40 μg / ml and cinnamaldehyde from 78 μg / ml to 20 μg / ml, both depending on the strain tested. In a second study, the effect of 0.4% cinnamaldehyde and 3% red propolis on unisex and multispecies biofilms developed on titanium surfaces was evaluated. The metabolic activity (MTT), cell viability (CFU / mL) and surface roughness (optical profilometry). In the first two trials the data were analyzed by ANOVA complemented by Tukey (α = 5%), after the profilometry analyzed descriptively. 0.12% chlorhexidine and 0.9% saline solution were used as positive and negative controls, respectively. Red propolis reduced the number of viable cells by 72% compared to the positive control group in the unicorn biofilm (p <0.05). In relation to metabolic activity, the red propolis group reduced 81% and 74% (p <0.05), the uniespecies and multispecies biofilms, respectively. Therefore, it is concluded that cinnamaldehyde, α-terpineol and red propolis present an inhibitory effect against Candida spp strains, although cinnamaldehyde has not demonstrated satisfactory activity in biofilms developed on titanium surfaces. Differing from the red propolis, which proved to be an alternative for the treatment of peri-implantitis.