Técnica para liberação do predador Euborellia annulipes (Dermaptera:Anisolabididae)

Abstract

With the increase in the excessive and inappropriate use of pesticides and the consequent negative effects on the environment, there is a need to search for alternative methods to chemical control of insect pests, biological control is one of the most relevant and most prominent methods in recent years. Among the beneficial insects with the greatest potential for use in this method are those of the order Dermaptera, which have stood out as important biological control agents of the main pests of large crops, notably the species Euborellia annulipes that has demonstrated its potential for use in biological control programs. The objective of this research was to evaluate the release technique of the predator E. Annulipes. The experiment was conducted at the Entomology Laboratory of the Federal University of Paraíba, Campus II. The release technique consisted of two factors: age of laying (one, three and six days after oviposition) and moisture in the release capsule (presence and absence), with ten repetitions of ten eggs for each treatment. Each posture was placed inside a biodegradable capsule, containing 2.0 mm diameter holes. After the insertion of the eggs and the closing of the capsules, they were stored in plastic containers (22.5 x 15.0 x 6.0 cm), containing an artificial diet and moistened cotton pad. Subsequently the containers were accommodated in B.O.D. with temperature of 25 ± 1ºC, relative humidity of 70 ± 10% and photophase of 12 hours. The rate of hatching of the nymphs and evasion (exit) of the nymphs from the capsules were evaluated. The evasion rate of E. annulipes nymphs from the capsule was assessed by observations every 12 hours for 12 days. On the fifteenth day, after the installation of the experiment, the capsules were opened and the hatching rate was evaluated. The data were evaluated using a generalized linear model for binomial data considering the beta distribution. In the treatment with six-day eggs, there was a higher probability of hatching, with higher values in capsule with the humid environment, also showing a higher probability of evasion, but without difference between the two environment conditions.