Influência de etil metano sulfonato em diferentes genótipos de pimenteiras ornamentais

Abstract

The pepper of the genus Capsicum, especially aa species Capsicum annuum L. brings together a variety of attributes such as color, shape and size of the fruits that make these stand out in the ornamental market. This fact drives the breeding programs and the creation of new varieties that meet the needs and market expectations. The induced mutation in genes is an alternative in this context. A chemical mutagen worth mentioning is the ethyl-methane-sulfonate (EMS), to be less phytotoxic. Given the above, the aim of this study was to evaluate the effect of EMS on the germination and development of seedlings and plants of different genotypes of ornamental pepper (Capsicum annuum L.). Seeds three genotypes of C. annuum were soaked in distilled, deionized water and autoclaved for 12 hours, then were subjected to different treatments Ethyl-Methane-Sulphonate (EMS), control and five concentrations (0, 0.5, 1 0; 1.5; 2.0; 2.5%). Subsequently, half of the seeds were inoculated in vitro while the other half were sown in polystyrene trays containing commercial substrate. They evaluated the percentage of germination and germination speed index (GSI). Subsequently, seedlings and plants have been characterized. Data were subjected to analysis of variance with subsequent comparison of means test. It was observed that different environments influence the germination and IVG and the different genotypes also behaved differently, but the EMS doses did not cause detectable variation on these characteristics. Likewise, the seedlings characteristics also varied according to different environments and genotypes, and the mutagenic agent was responsible for variation only in hypocotyl length and diameter. For plants, the three factors (doses, environments and genotypes) were individually responsible for significant changes to various features. The three factors (doses, environments and genotypes) were individually responsible for significant variation in various characteristics. EMS generated variability, but new mutagenic doses should be tested.