Estudo in vitro da eficácia de nanopartículas de quitosana encapsulando óleo de pequi (Caryocar coriaceum) frente a Enterococcus faecalis

Abstract

Enterococcus faecalis is a microbial species commonly isolated in persistent periapical infections. The intracanal medication aims to reduce the residual microbiota, and, in this way, it becomes important to research new materials for its incorporation. Aim: to study in vitro the efficacy of chitosan nanoparticles with Pequi (Caryocar coriaceum) oil encapsulated against E. faecalis. Methodology: C. coriaceum oil was extracted from the fruit mesocarp using the rotavaporation technique. Chitosan was obtained by means of sequential stages of pre-treatment, demineralization, deproteinization, deodorization and drying. Chitosan nanoparticles were obtained through deacetylation followed by neutralization and dripping of sodium tripolyphosphate (TPP). Results: No antimicrobial activity of C. coriaceum oil was observed in any of the tested concentrations: 3.88 mg / mL chitosan, 2, 34 mg / mL oil, 3.834 mg / mL chitosan, 3.82 mg / mL chitosan, and 0.017 mg / mL oil. Thus a new Disk Diffusion test was performed with a pure sample and a higher oil concentration (10 μl), which confirmed the antimicrobial inactivity. Conclusion: It was concluded that the oil of C. coriaceum encapsulated in chitosan nanoparticles did not exert antimicrobial activity on E. faecalis.