Atividade antimicrobiana do cinamaldeido e α-terpineol frente biofilmes endodônticos

Abstract

Objective: To evaluate the antimicrobial activity of cinnamaldehyde and α-terpineol against endodontic biofilms. Methods: Bovine Root canals were instrumented with K (#45 to 60) with filing and enlargement movements, under constant irrigation with distilled water, and smear leayer removal with 17% EDTA. Afterwards, they were sterilized by ethylene oxide and a biofilm cultivated in the channels. The cell concentration used for E. faecalis was 1x108 CFU / mL in BHI medium and the culture was performed for 14 consecutive days (37°C, microarephilia). On day 15 the biofilms were exposed to cinnamaldehyde (Cin) and α-terpineol (Terp) at 10 mg / mL for 16 min, at 37°C. BHI medium was added and the samples reincubated for 24h. BHI medium solutions were used as negative control; sodium hypochlorite (NaOCl) and chlorhexidine (CHX), at 2%, as a positive control. The biofilms were analyzed according to the cellular viability (CFU/mL) (n=9/group) and the total protein concentratio (n=8/group). The Kruskal Wallis and Mann Whitney tests were applied for cell viability and ANOVA and Tukey for the determination of total proteins (α=5%). Results: For the UFC/mL count, the CH showed the same CHX metabolism (p>0.05), both differed from the negative control, Terp and NaOCl (p<0.05). There was only difference in the production of total proteins between the negative control and Cin (p <0.05). Conclusion: Cinnamaldehyde was the substance that presented antibacterial effect against endodontic biofilm comparable to chlorhexidine 2%.