Avaliação da toxicidade in silico e das atividades hemólitica, antioxidante e antibacteriana in vitro das formas óleo essencial e óleo essencial microencapsulado de lippia pedunculosa.

Abstract

Since the existence of humanity the resources of the vegetable kingdom are of undeniable importance for the development of society. Modern, welldeveloped medicine, most of the world's use of medicinal plants in primary health care. Faced with this great and professional and academic interest in researching with natural products as to their pharmacological, toxicological activity, their chemical and botanical characterization in order to regulate their demands regarding the quality efficacy and safety to then reach clinical trials and development of drugs. Studies show that essential oils of L. pedunculosa present amebicidal activities, repellent against larval form of Ae. Aegypti, analgesic in the models of chemical nociception, as well as anti-inflammatory capacity. Due to better application and durability of the essential oils form and developed encapsulation techniques, as advantages offered by microencapsulation processes allow better efficiency of some nonpolar countries. Just as it is extremely important to evaluate the biological action in the screening of a vegetable, it is also the toxic tests, which adds information to ensure proper safety in its application. In silico and in vitro assays, the cytotoxicity in human erythrocytes was investigated, investigating the oxidant profile and antioxidant properties in the presence of reactive oxygen species and oxidizing agent, and determine the Minimum Inhibitory Concentration (MIC) against Gram-positive bacterial strains and Gram-negative diseases of clinical importance. OELP met the requirements recommended by -Law of the five of Lipinski, presenting in the in silico approach, in the in vitro tests showed strong hemolytic activity and limited antihemolytic activity. OELP-ME induced low hemolysis and protected erythrocytes in the presence of hypotonic solution (NaCl 0.24%). OELP as well as OELP-ME did not induce hemoglobin oxidation, however, it did not present an antioxidant effect in the presence of phenylhydrazine. However, OELP-ME presented an antioxidant effect against hydrogen peroxide. As for the antimicrobial activity, only OELP has been shown to be effectively active against microorganisms with a proven bacteriostatic effect.