Avaliação de um condicionador tecidual contendo terpinen-4-ol e cinamaldeído anti-Candida albicans

Abstract

Introduction: Denture Stomatitis it is a injury multifactorial what occurs at the palate under a prosthesis removable. Has as main factor associated the infection for Candida albicans. The incorporation in agents antifungals in tissue conditioners for relining the prosthetics has been a alternative for the reduction of development in biofilms adherents on their surfaces. Terpinen-4-ol and Cinnamaldehyde are phytoconstituents of essential oils with have several properties, especially antifungal effects and potential use in conditioners. Objective: to compare in vitro antifungal efficacy of phytoconstituents in conditioner Softone and the effect of such modification in Shore A hardness. Methodology: The Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of phitoconstituents were determined by microdilution and plating of subcultures. The same technique was used to verify if the phitocontituents interact with the fungal cell wall or membrane, in the presence of sorbitol and ergosterol, respectively, thus determining the mecanism of action. The method of diffusion in agar was used for antifungal evaluation of isolated phitoconstituents or incorporated into the conditioner at different concentrations (0.0156; 0.125; 0.25; 0.5; 1; 5;10; 20, 30 e 40%) and periods (24, 48h, 4 e 7 dias). In biofilm the viability cell was determined spectrophotometrically in reduction of salt in tetrazolium (TTC) after 24 and 48h At the same concentrations in the above assay. Hardness tests in durometer Shore A were carried out on the specimens modified by phitoconstituents (5, 10, 20, 30 and 40%) after immersion in artificial saliva at 37°C in periods (baseline, 24, 48h, 4 and 7 days).. The Kruskal-Wallis and Wilcoxon (α=0.05) was used in the disk diffusion assay. To evaluate the hardness was used Analysis of Variance (ANOVA) and Tukey post test (α=0.05). Results: The values of MIC and MFC were 1.125μg / ml and 2.250μg / ml to Terpinen-4-ol and 156μg/mL and 156μg/mL for Cinnamaldehyde, respectively. Essays on the mode of action suggests that Cinnamaldehyde acts causing damage to the membrane and the wall of the fungal cell. Terpinen-4-ol seems to have no activity on fungal cell structure this study. In agar the phytoconstituents have not shown significant antifungal activity until the conditioner concentration was increased above 5%. Cinnamaldehyde and Terpinen-4-ol (20-40%) when mixed with the conditioner, completely inhibited fungal growth. The reduction assay ttc Demonstrated that incorporation of 10 Cinnamaldehyde (10-40%) effectively inhibited biofilm formation in both periods and, modified conditioner Terpinen-4-ol showed viable cells even at the maximum concentration (40%). The addition of the phytconstituents in Softone resulted in Increased hardness of the materials during the evaluation period, however, this variation is considered acceptable and non interfering with the clinical use of modified conditioner in seven days. Conclusion: The incorporation of Terpinen-4- ol and Cinnamaldehyde in tissue conditioner can be considered a promising therapeutic modality for denture stomatitis, with the advantage of less active Involvement of the patients.