Estudo de polimorfismos nos genes da IL-17A e IL-17F em portadores de psoríase

Abstract

Psoriasis (PsO) is a chronic immune-mediated inflammatory disease characterized by proliferation of keratinocytes and formation of skin plaques. Th17 lymphocytes and their cytokines IL-17A and IL-17F are widely described as essential in the pathophysiology of the disease. In addition, several single nucleotide polymorphisms (SNPs) have been reported to be responsible for different clinical manifestations of the disease. Due to the few clinical and genetic studies on PsO in the Americas, including Brazil and the Northeast region, the objective of this study was to evaluate the correlation between SNPs in the IL-17A (rs2275913 and rs3819024) and IL-17F (rs763780 and rs2397084) genes with the PsO in patients seen at the Lauro Wanderley Universitary Hospital of the Federal University of Paraíba. Blood samples were collected from 107 patients and 55 controls, from which DNA extraction and subsequent SNPs genotyping were carried out using the real-time PCR technique using the TaqMan probe-based 5 ́-nuclease assays technology. Statistical analysis were performed using R, coin and SNPassoc software, applying two-tailed hypothesis tests with a 5% significance level. The case-control analysis adjusted for sex and age showed that SNPs rs763780 (OR = 14.8; CI = 1.77-123.3; p = 0.0127) and rs3819024 (OR = 4.98; CI = 1.02-24.4; p = 0.0478) were associated with the risk of PsO occurrence while rs2275913 (OR = 0.02; CI = 0.0004-0.93 ; p = 0.0459) was associated with protection against PsO. These results suggest that the study of the patients' genetic profile may be an innovative approach to better elucidate knowledge about PsO pathogenesis and immunotherapy.