Memória e ritmicidade circadiana em ratos adultos submetidos a um protocolo de dessincronização forçada durante as fases de infância e adolescência: Modelo T22

Abstract

Learning and memory problems related to disruption of circadian rhythms are evident among children and adolescents with access to electronic devices and irregular hours of wake and sleep. Being essential for the functioning and regulation of several metabolic, physiological and behavioral functions, the study of possible effects on the dysregulation of this system is of great importance for the wellfare of living beings. This research used the T22 forced desynchronization protocol (1 1 light and 11 dark) to induce a disruption of circadian rhythmicity from locomotive activities and to evaluate damage in recognition memory , spatial memory and rearing behavior in adult animals submitted to the protocol in childhood or in adolescence. W e used 40 males Wistar rats, separated into 3 groups: control group (16 animals), which remained at T24 throughout the experimental period; group that underwent pregnancy dissociation on the 21st day of birth (Childhood D - 12 animals); and group under dissociation from weaning on the 61st day of birth (Adolescence D - 12 animals). These rats performed in adulthood, under T24, behavioral tests of memory: object recognition task (OR T) and associative spatial recognition (ASR). Analysis of the actogram shows that the T22 protocol promotes disruption of the locomotive of progenitress in the Childhood D group and in the animals of the adolescents D group, which means the animals had two rhythms of activity . Behavioral tests showed an impairment in the OR T and ASR tasks for CD, while for AD the performance in ASR is impaired, indicating differential maturation in different brain structures responsible for different types of memory . This study shows that circadian desynchronization can cause damage to different types of memory and these impacts can persist until adulthood.