Investigação da ação do trans-anetol em modelos comportamentais de convulsão em camundongos

Abstract

Epilepsy is a chronic,not transmissible and treatable disease.Can it be characterized by electrical discharges than afecct a region of the brain or several, occurring in a way recurrent and unprovoked. The pharmacoresistance and the adverse effects of anticonvulsants boost studies.The essential oils from the plants and with inherent anticonvulsant activities are promising therapeutic alternatives. The trans-anethole (TAN),is major constituinte of essential oils from anise,green anise and star anise.It is a substance used as flavoring and adjuvante in foods and with previously proven anticonvulsant activity.The objective of this work was investigate a possible mechanism of action of TAN via the in sílico (Docking Molecular) method, associating with is possible anticonvulsant activity in vivo at doses 50, 100 e 150 mg/kg (i.p),using the convulsant chemical agentes pilocarpine and 3- mercaptopropionic acid,as well as to evaluate the synergism of the TAN Against the classic anticonvulsants.For this,male Swiss mice (Mus musculus) were used,protocols approved by the Animal Ethics Commission (CEUA) of the Federal University of Paraíba, under the certificate number 3890250918. In sílico studies using molecular docking methodology observed an affinity for receptors GABAA and N-metil D-Aspartato (NMDA). In vivo studies,using the chemical agent pilocarpine 350 mg/kg (i.p),observed anticonvulsant activity of TAN at the dose of 100 mg/kg (i.p) (15,5±4,4 s),in the parameter duration of the crisis when compared to the vehicle group (25,8±10,3 s). In the observation of seizures with the 3- mercaptopropionic acid 40 mg/kg (i.p), the dose of TAN 50 mg/kg (i.p) [14,5, (13,4-14,5 s)] and TAN 100 mg/kg (i.p) [11,27(9,57-14,75 s)] reduced the duration of generalized tonic clonic seizures when compared to vehicle [22,4 (21,3-22,4 s)]. When TAN was combined at its subconvulsant dose 150 mg/kg (i. p), with phenobarbital (20 e 40 mg/kg i. p) and phenytoin (12,5 mg/kg e 25 mg/kg i. p) there was a reduction in the duration of tonic extension of the hind limbs of the animals.As well as a reduction in mortality when compared to the vehicle group.From the in sílico results it can be predicted that trans-anethole acts via receptors GABAA and NMDA and the in vivo results developed demonstrate the anticonvulsant activity of TAN and its beneficial associations with the classic anticonvulsants combined with a possible neuroprotective effect.