Estabilidade do RNA do SARS-CoV-2 obtido de amostras de saliva ao armazenamento por longo período

Abstract

Viruses are obligate intracellular microorganisms, among which the SARS-CoV-2 virus, responsible for the Covid-19 pandemic, is included. During this period, early diagnosis has become imperative to initiate treatment measures promptly. Real-time reverse transcriptase polymerase chain reaction has been established as the gold standard for testing this virus. The primary specimens used are nasopharyngeal and/or oropharyngeal swabs, but saliva has also been employed as a sample for viral detection. Considerable discourse persists concerning SARS-CoV-2, including discussions on the stability of viral RNA. This study aims to scrutinize the literature on the stability of SARS-CoV-2 RNA extracted from saliva samples and stored for extended periods. Following an extensive search across databases and the application of predefined descriptors, we initially identified 239 articles. After filtering them through inclusion and exclusion criteria, only six remained. Our findings indicate that viral RNA can maintain stability for a range of three to ninety-two days, contingent upon the storage conditions of the samples. However, it is noteworthy that the literature lacks investigations into longer periods, such as months or years, regarding the analysis of its stability