Potencial anti-hemorrágico e anti-hialuronidase de produtos naturais e derivados contra a peçonha de Bothrops erythromelas

Abstract

ABSTRACT: Snakebite represents a serious health problem in the tropics. In this context, the problem of snakebites goes beyond the numbers related to mortality, and is related to a serious sequelae, which makes daily life impossible for many of the victims. The Bothrops erythromelas snake is the main responsible for snakebites in the caatinga biome, but this species is not included in the pool used in the production of antivenom. Considering the limitations of antivenom, several studies have been carried out to develop complementary treatments for snakebite and, in this context, plants constitute an important source of bioactive compounds. Thus, it was proposed to evaluate the capacity of crude extracts of Doyerea emetocathartica, Cnidosculus quercifolius and rosmarinic and chlorogenic acids to inhibit the hemorrhagic activity, as well as the hyaluronidase activity of Bothrops erythromelas venom. Initially, the Prothrombin Time and Activated Partial Thromboplastin methodologies were used to evaluate the integrity of the in vitro coagulation cascade. Then, to measure the preservation of hyaluronic acid in vitro, the turbidimetric method was applied. The results obtained demonstrated significant inhibition activity at all concentrations in reducing prothrombin time with extracts of Doyerea emetocathartica and Cnidosculus quercifolius (93.3% and 94.01%) and rosmarinic and chlorogenic acid (94.5 % and 94.01%). It was also effective in reducing activated partial thromboplastin time in D. emetocathatica and Cnidosculus quercifolius (95.16% and 95.14%) and rosmarinic and chlorogenic acid (96.02% and 95.52%). When evaluating the antihyaluronidase potential, all compounds of plant origin were able to maintain the integrity of the substrate, Doyerea emetocathartica and Cnidosculus quercifolius (2,811 ABS and 1,819 ABS) and rosmarinic and chlorogenic acid (0.5 ABS and 1.57 ABS). Given this scenario, the results demonstrate that the extracts are good candidates for the adjuvant treatment of snakebites caused by Bothrops erythromelas, and may also be promising for research aimed at the independent development of anti-hemorrhagic drugs and anti-inflammatory potential.