Isolamento, identificação e caracterização de bactérias lácticas na ensilagem de palma forrageira

Abstract

The objective of this study was to isolate, identify and characterize the strains of lactic cultures in forage palm (Nopalea cochenillifera Salm Dyck cv. Miúda) ensilage from the fermented juice. The populations of lactic bacteria were quantified before and after of the forage palm ensilage using selective culture medium. The obtained data were grouped and discussed through descriptive statistical analysis. The isolates from the silages were classified as gram positive, negative catalyses and bacilli form. Thirty-nine strains were cultivate in MRS broth and selected twenty strains based on their lactic and acetic acid production capacity. The lactic and acetic acid concentrations varied of 1866.62 to 186.98 and 594.60 to 46.38 mg/dm3 respectively. It was observed a microbial diversity in forage palm ensilage with predominance of heterofermentative bacteria (Weissella) and homofermentative bacteria (Lactobacillus plantarum) in the plant. The bacteria specie Weissella cibaria was featured by produce in high amount of acetic acid and remained in the silage after the fermentation period, when compared with others. Lactobacillus plantarum was the homofermentative specie predominant in the forage palm silage.