Modulação da microbiota intestinal de codornas europeias por meio do uso de enzimas exógenas

Abstract

Changes in intestinal microbiome of birds can be promoted through the diet, so the constituents of the diet deserve attention about their effects on the intestinal ecosystem, among them fiber and the use of enzymes have a capacity to modulate the microbiota. The objective of this study is to analyze the modulation of the microbiota of European quails diets based on corn, soybean meal and wheat bran with or without the use of xylanase and phytase.224 European quails were used, distributed in a completely randomized design with eight treatments, seven replications with four birds per experimental unit. The treatments consisted of: Diet 1 based on corn + soybean F. (T1); Diet 1+ xylanase (T2); Diet 1 + phytase (T3); Diet 1 + phytase + xylanase (T4); Diet 2 based on wheat+ soybean F. (T5); Diet 2 + xylanase (T6); Diet 2+ phytase (T7); Diet 2+ phytase+ xylanase (T8). At 42 days, ten birds from each treatment were slaughtered to collect the intestinal contents after collection, a pool was performed, where the samples were identified, frozen and stored in a falcon tube and sent to the Neoprospecta Microbiome Technologies laboratory for analysis of the microbiota.The microbial profile was affected by dietary changes and enzyme supplementation, in both diets without enzyme supplementation there was a greater predominance of the genera Streptococcus and Lactobacillus and the species Lactobacillus agilis, Streptococcus macedonicus and Lactobacillus salivarius, however, the use of xylanase in the maize diet had a predominance of S. macedonicus followed by L. agilis and L. salivarius, however in the wheat bran diet there was a predominance of L. agilis and L. salivarius species, the use of phytase in the Maize diet showed greater dominance of L. agilis, when replacing corn with wheat bran, there was dominance of S. macedinicus, however, the association of the two enzymes provided a greater amount of L. salivarius and L. agilis with different proportions between the Maize or Wheat bran based diets. The use of wheat bran with the use of phytase and xylanase modulates the European quail microbiota, with an abundance of specific bacteria (L. agilis, L. salivarius and S. macedonicus) and dominance of lactic acid bacterias.