AVALIAÇÃO DO EFEITO DO TEMPO, TEMPERATURA E USO DO FLUORETO DE SÓDIO EM AMOSTRAS DESTINADAS A DOSAGEM DE GLICOSE SANGUÍNEA EM GALINHAS (Gallus gallus domesticus) E CODORNAS JAPONESAS (Coturnix coturnix japonica)

Abstract

Laboratorial tests of blood are important tools for birds medical clinic, and glycemia dosage is one of the most common biochemical tests used. In most part of animal species, glucose concentration reduction in vitro is related to the glycolysis activity of the erythrocytes. In birds, on the other hand, that does not happen due to fatty acids use by these cells as energy source. In spite of hematology and biochemistry of birds being similar to human and other mammals, some particularities as the presence of nucleated red blood cells, thrombocytes, heterophile granulocytes and different erythrocyte metabolism make modifications on laboratorial procedures needed. There are few studies clarifying the basic aspects linked to the physiology and clinical-laboratorial evaluation of these animals. This paper aims to evaluate time effect, temperature and use of sodium fluoride in samples intended for blood glucose dosage in chickens (Gallus gallus domesticus) and japanese quails (Coturnix coturnix japonica) by calibrating glucose in plasma and serum samples, separated in different time intervals, and incubated at 37ºC and at room temperature. It was determined the glucose concentration means and standard deviations of blood samples with sodium fluoride without anticoagulant, separated by time and temperature. It was also observed relative average consumption and cumulative average consumption rates of each time interval. It was verified a lower average concentration (p<0,05), and a higher relative and cumulative average consumption of glucose (p<0,05) in plasma samples with sodium fluoride than in serum samples, kept at room temperature and incubated at 37ºC, during different times. Thus, this paper enhances the sodium fluoride ineffectiveness on preserving glucose in vitro, besides a probable catalytic effect on the lysis of this nutrient.