RESISTÊNCIA DE PIMENTEIRAS ORNAMENTAIS (Capsicum annuum L.) A NEMATOIDES DAS GALHAS ACESSADA POR MARCADOR MOLECULAR ISSR

Abstract

Peppers belong to the genus Capsicum and are appreciated and cultivated worldwide. The ornamental pepper trade has been growing due to its great diversity related to several characteristics of interest, such as: small size, colorful and showy fruits with different colors during the maturation process. One of the problems that reach this crop and that causes damages is the root-knot nematode, whose is control most indicated is the use of resistant plants. Therefore, this work aimed to evaluate the resistance of different accesses of Capsicum to root-knot nematodes under the use of the ISSR molecular marker. For the present study, DNA from young leaves of the accesses AC04, AC05, AC06, AC07, AC09, AC10, AC11, AC13, AC23, AC24, AC25, AC26, AC27, AC28, AC29 and AC30 was extracted and amplified by ISSR-PCR using 9 primers. The reaction products of each primer were compared with the specific markers of resistance to root-knot nematode reported in the literature. Among the 16 accesses evaluated, a possible resistance to root-knot nematode was observed in AC05, AC07, AC09 e AC28 accesses. The primers LA-7 e LA-8 are possibly associated with N nematode resistance gene. However, it is necessary to confirm the results reported in this work with the inoculation of nematode eggs of different species that attack peppers.