Microfabricação de um analisador em Fluxo-Batelada (Micro Flow-Batch) à base de polímero fotocurável Uretano-Acrilato

Abstract

This work describes the construction of a new flow-batch analyzer (FB) in micro scale (micro Flow-Batch-μFBA) using the technique of deep ultraviolet photolithography with commercial resin based in urethane acrylate oligomers (UA). The device was constructed from the union of two layers of pre-polymerized resin of 3.4 mm thick each, thus forming a single structure. The main feature of the new device is the small volume of its analysis chamber, which can be 100-200 μL, allowing the homogenization of reagent solutions in a time of 2.s. In this sense, the decrease in reagents consumption and hence generation of waste (ten times smaller than a conventional FB), wich is in line with the requirements of green chemistry and with the new position of the eco-efficiency. The system offers the following progress in relation to micro fabrication with urethane acrylate resin: (i) depth control of the channels during photolithography; (ii) axial mixer incorporated; (iii) LED - Light emitting diode (530 nm) and detector (photodiode) coupled to the device body; The depth control of the channels allows adjustment of the volume of the chamber and the LED to the mixing chamber. The LED and detector connector pins is the elements that makes the device fixed on its box. In this first assembly, system were used in photometric determination of Fe (II) in pharmaceuticals. The model for the calibration curve was validated by analysis of variance (ANOVA), and their analytical results were compared with those obtained in batch by the reference method, the application of paired t-test found no statistically significant differences at a confidence level of 95%.