Atividade antimicrobiana in vitro de uma esponja marinha (cinachyrella sp.) Sobre patógenos orais

Abstract

The most common oral infections are dental caries, periodontal disease and candidiasis. Currently, pharmacologically active products are available in the market to combat the microorganisms that cause these pathologies; however, the increase in microbial resistance and undesirable side effects, makes the search for products of natural origin grow. The marine environment represents an enormous and still unexplored source of natural products, with a high structural and pharmacological diversity. Studies on this biodiversity have verified that the representative with the largest source of molecules with bioactive potential are the marine sponges. In this way, the present study aimed to evaluate the in vitro antimicrobial effect of the crude ethanolic (EtOH), hydroalcoholic and chloroform (CHCl3) extract of the Cinachyrella sp. on oral pathogens. The minimum inhibitory concentration (MIC) was evaluated by the microdilution technique in 96-well plates and the minimum fungicidal (CFM) and minimal bactericidal (MBC) concentrations by sowing the subculture, with nystatin and chlorhexidine being used as positive controls. This species exhibited a satisfactory antifungal potential in strains of Candida albicans CBS 562, Candida tropicalis CBS 93 and Candida Krusei CBS 593 and on the bacterial strain of Streptococcus sanguinis, with emphasis on the CHCl3 phase,