Prospecção e uso de culturas lácticas como inoculante na ensilagem de palma forrageira

Abstract

This study involved two experiments. In the first, the objective was to select strains of lactic acid bacteria (BAL) isolated from the plant and cactus pear silage and to evaluate its effects on silage fermentation and aerobic stability. Forty isolates classified as BAL were isolated from the plant and forage palm silage and evaluated for the production of metabolites and identified by 16S rDNA sequencing. Isolates were identified as Lactobacillus plantarum, Weissela cibaria, Weissella confusa and Weissella paramesenteroides. The population of lactic acid bacteria (BAL), water soluble carbohydrates (WSC) and ammoniacal nitrogen differed (P <0.001) among the silages. Enterobacterial, yeast and fungal populations were below the minimum detection limit (<2.0 log cfu / g silage) in all silages studied. The predominant action of the inoculants was the maximization of the recovery of DM of the silages, which could be the criterion adopted to select the BAL strains for use as inoculants in the Nopalia silages. In the second experiment, the objective was to compare the in natura and ensiled cactus pear with and without microbial inoculant in the sheep diet, through the evaluation of the consumption, nutrient digestibility, nitrogen balance and microbial protein synthesis, as well as fecal enterobacteria count. Twenty sheep with no defined racial pattern were defined, non-castrated at approximately 6 months of age and mean initial weight of 23.48 ± 2.40 kg. These were distributed in a completely randomized design with four treatments (forage palm in natura processed twice a day at the time of supply (PF1); fresh cactus pear processed once only in the morning and supplied twice a day (PF2) (SPI) and silage (SP and SPI) resulted in a higher number of silage species (SPI and SPI), as well as in silage (SP) and cactus silage with microbial inoculant (SPI) and five replications. P <0.05), dry matter intake, organic matter, neutral detergent fiber, ethereal extract, non-fibrous carbohydrates and total digestible nutrients, higher digestibility coefficients of dry matter, organic matter, as well as total digestible nutrients, On the other hand, there was no effect (P> 0.05) of the diets on the nitrogen balance, mic efficiency robiana, losses of urinary nitrogen, faecal and microbial efficiency. The use of forage palm silage promotes a reduction in the enterobacteria count, improving nutrient intake and digestibility.