Utilização da água de coco em pó, diluidor de Kenney, leite UHT e Tris-gema de ovo na criopreservação de sêmen de cães.

Abstract

Studies focused on methodology development for semen quality preservation are fundamental in the improvement of artificial insemination techniques, which is an important procedure for genetics improvement and endangered species maintenance. The objective was to evaluate the quality of canine semen refrigerated with four extenders: ACP-106®, Kenney, skim UHT milk and egg-yolk Tris. There were used semen samples from three adult mixed-breed dogs, harvested at weekly intervals and divided into four sections, each one diluted in each type of extender, and, finally, refrigerated. The progressive motility, vigor and funcionality of the plasma membrane were evaluated every 12 hours after refrigeration until the samples presented viability. The results obtained demonstrated that the Kenney extender has a better use in regards to it’s progressive motility, vigor and funcionality of the plasma membrane, wich presented the best viability at the longest time interval analysed (48 hours), however ACP-106® and skim UHT milk proved to be viable alternatives until up to 12 hours after refrigeration.