Sistema para detecção de peçonha ofídica a partir de um sensor de ressonância de plasma de superfície

Abstract

Snakebites are a neglected tropical disease that affects a significant number of people each year worldwide. The production of antiophidic serum, rapid diagnosis, administration and efficacy are fundamental steps for the good evolution of patients diagnosed with snake envenoming. In Brazil, snake accidents victimize about 20,000 people annually, with botropic accidents being the most common across the country, followed by crotalic accidents. Several species of the genus Bothrops can be found in the Northeast, with B. erythromelas being the most important from a medicalepidemiological point of view. In view of this, this work aimed at a method for the development of a biosensor to detect snakebite poisoning, testing the venoms of the species Bothrops erythromelas and Crotalus durissus terrificus, using an optical surface plasma resonance sensor - SPR. For this, the analyte and the adsorvate were evaluated, the latter, F(ab')2 fragments of IgG from the antivenom, titrated in different concentrations in order to evaluate their interactions. Detection tests were performed on the SPR biosensor following optimization parameters for better adsorption in the gold chip. The results showed that the sensor is able to differentiate the poisons of the two snake species used in this study. It can be concluded that the development of a biosensor to detect the type of snake venom is possible through this sensor. These data are of paramount importance, since there is a great need for differentiation when the poisoned individual is admitted to the hospital for treatment.