Avaliação da atividade antifúngica in vitro da molécula 2-bromo-N- fenilacetamida contra isolados de Candida glabrata oriundas da cavidade bucal

Abstract

Objective: To evaluate the antifungal potential of 2-Bromo-N-phenylacetamide (A1Br) against oral cavity C. glabrata; Materials and methods: An in vitro laboratory study using an inductive approach was performed. Assays to evaluate the antifungal activity of A1Br, nystatin and miconazole against C. glabrata strains were performed using microdilution technique. Minimum Inhibitory (MIC), and Minimum Fungicide (MFC) Concentrations were determined, as well as evaluation of yeast structures and association tests of the molecule with standard antifungals. Results: The A1Br molecule presented an MIC of 16 μg/mL and an MFC of 32-64 μg/mL; revealing antifungal potential similar to nystatin (MIC 8 μg/mL and MFC 8- 32 μg/mL) and superior to miconazole (MIC 64 𝜇g/mL and MFC of 512-1024 𝜇g/mL). The A1Br - nystatin association presented antifungal effect. The A1Br - miconazole association presented fungistatic effect. Micromorphological evaluation revealed decreases in all structures for the two studied strains at the antifungal concentration of A1Br =MIC x 2, reaching 100% at A1Br = MIC x 4. The association study with nystatin revealed antagonism (FICI = 4.5) and with miconazole revealed indifference (FICI = 1.25). Conclusion: The studied molecule presents excellent antifungal effect against C.glabrata. Clinical Relevance: The search for new products and formulations is based on a great necessity for more alternatives against the fungi which cause oral C.glabrata infections. Despite the excellent antifungal activity potential of the studied molecule, further studies are needed to evaluate mechanisms of action and associations with other substances.