Desenvolvimento de uma metodologia voltamétrica para determinação de aminas biogênicas usando eletrodo de carbono vítreo modificado com pontos quânticos de carbono

Abstract

Biogenic amines (BAs) are nitrogenous compounds that have a low molecular weight, are formed from the degradation of nitrogenous compounds by the action of enzymes and can be associated with the process of deterioration or fermentation of food. Currently, there is an interest in the study of biogenic amines, due to the toxicological activity that some of these compounds can present to humans. Histamine (HTM) is associated with food poisoning problems and can cause allergic reactions, such as shortness of breath, vomiting, fever, hypertension, etc . While tryptamine has toxic effects, promoting an increase in blood pressure. The litera ture reports the use of chromatographic methods for the determination of BAs in several matrices, but some inconveniences found in the development of these methods make it necessary to search for alternative methods for the determination of these compounds. The main objective of the present work is the use of square wave voltammetry (SWV) using a glassy carbon electrode (GCE) modified with carbon quantum dots (CQDs) for the determination of tryptamine and histamine under physiological conditions (pH 7). The methodology used for the synthesis of nanoparticles was carried out from the bottom-up approach, which is already wellfounded in the literature. The modification of the GCE consisted of performing 8 consecutive scans in cyclic voltammetry, at a scan speed of 50 mV s -1 , using a mixture of carbon dots, β-Cyclodextrin and phosphate buffer pH 7. The use of the modified electrode with carbon dots presents better responses for the simultaneous measurement of tryptamine (12,5 – 25,0 μmol L -1 ) and histamine (25,0 – 50,0 μmol L -1 ), with limits of detection and quantification of 0,2529 μmol L -1 and 0,8429 μmol L -1 for tryptamine and detection and quantification limits of 0 ,6789 μmol L -1 and 2,263 μmol L -1 for histamine, respectively.