Associação entre periodontite e lúpus eritematoso sistêmico: investigação de aspectos clínicos e genéticos

Abstract

The association between periodontitis and SLE has been primarily based on the similar pathophysiology. However, there are controversial data concerning periodontal status in SLE patients, as well as the underlying mechanisms of PD and SLE association are still largely unknown. Aim: Considering the influence of environmental and genetic factors in the pathogenesis of inflammatory diseases, this study investigated the contribution of the methylation-related gene polymorphisms DNMT3B (rs2424913) and MTHFR (rs1801133) to periodontitis and SLE. Methods: Overall, 196 individuals of both sexes were allocated into four groups under assessment of systemic and periodontal status as follows: healthy control (n = 60), Periodontitis (n = 51), SLE (n = 47) and SLE + Periodontitis (n = 38). Individuals with SLE were stratified according to disease activity (SLEDAI) in mild or severe. Polymorphism analysis was performed through PCR-RFLP using genomic DNA from oral mucosa cells. The data were analyzed by Fisher's exact, chi-square test and regression models. Results: Periodontal status was similar in subjects with periodontitis and with SLE + periodontitis. SLE patients with periodontitis presented a longer SLE diagnoses in comparison to SLE only (P=0.001). For DNMT3B polymorphism, Periodontitis, SLE and Mild SLE + Periodontitis groups showed a higher frequency of T allele and TT genotypes compared to healthy controls (P<0.05). Regression analyses showed the TT genotype increases the odds of periodontitis (6- fold) or SLE (11- to 12-fold). Conclusion: SLE patients with periodontitis had a longer length of SLE. The DNMT3B (rs2424913) polymorphism was associated with periodontitis, and SLE alone or combined with periodontitis.