Prospecção de metabólitos secundários das partes aéreas de erythroxylum rimosum o. e. schulz (erythoxylaceae)

Abstract

The wide use of plant species for therapeutic purposes is due to the fact that plants are sources of natural phytochemicals and, in order to maintain their internal homeostasis, they synthesize and store a variety of metabolites. Erythroxylum rimosum O. E. Schulz is a sub-shrub with records in the States of Piauí, Ceará, Rio Grande do Norte, Paraíba, Pernambuco and Sergipe. Previous studies with E. rimosum reported the presence of pentacyclic triterpenes, steroids, tropic alkaloids and flavonoids. However, studies about this species are still very scarce. In this context, a phytochemical study of the crude ethanol extract (BSE) from the aerial parts of E. rimosum was carried out. The plant material was collected in the municipality of Pirambu, state of Sergipe and identified by Profa. Dr. Ana Paula do Nascimento Prata, from the Department of Biology at the Federal University of Sergipe (UFS). To obtain the BSE, the material was dried in an oven, crushed and submitted to maceration with 95% ethanol (EtOH). The crude ethanol extract was dissolved in a methanol:water (7:3 v/v) solution and partitioned with hexane, dichloromethane and ethyl acetate solvents. The ethyl acetate phase was subjected to column chromatography, using silica gel as stationary phase and as mobile phase, the solvents hexane, ethyl acetate and methanol. Thirty fractions were obtained and pooled according to their respective retention factors (Rfs). The pooling of fractions from 6 to 7, coded as Eri-1, was analyzed by Nuclear Magnetic Resonance (NMR)1H,13C-apt and compared with literature data, being identified as Catechin, already isolated in the species under study. . The Hexanic phase was subjected to medium pressure chromatography using silica gel as stationary phase and, as mobile phase, the solvents hexane, chloroform, ethyl acetate and methanol. 86 fractions were obtained that were pooled according to their RFs. The pool of fractions 23 to 26, coded as Eri-2, was submitted to the same methods of analysis as Eri-1, being identified as 3-oxo-friedelane, reported for the first time in this species.