Produção de proteases usando Penicillium sp. FSDE15 em resíduos agroindustriais

Abstract

The enzyme market has been growing especially because the huge diversity among industrial fields. The production of these molecules with high specificity and stability at pH, temperature and chemicals linked to low cost substrates has been constantly researched. Therefore, the aim of this study was to evaluate the production of proteases from the fungus Penicillium sp. FSDE15 by solid-state fermentation with agroindustrial residues. The experiment performed to obtain proteolytic kinetics used wheat bran and malt bagasse to find out the most successful substrate for this process. It was also realized the quantification of cellulolytic and amylolytic activities to verify the performance of these enzymes under the related conditions. The fermentation has lasted 7 days, and enzymatic extractions were performed using sodium phosphate buffer 50 mM, pH 7.4 for proteases analysis and sodium citrate buffer 50 mM, pH 4.8 for cellulases e amylases analysis for 3 hours under continuous stirring. Through the fermentative tests it was possible to reach a maximum production of proteases and cellulase of 26.42 ± 1.20 U/mL and 0.141 ± 0.001 U/mL, respectively, using extract from dried growth at 40 ºC for 48 hours. The maximum production of amylases of 3.080 ± 0.103 U/mL occurred at 168 hours of experiment and it was not verified higher values using extract produced through dried growth. These results show the enzymes produced by Penicillium sp. FSDE15 as potential additives for animal feed and as components of enzymatic detergents.