Isolamento, cultivo e criopreservação de células obtidas a partir de fezes de caprinos: Um modelo experimental para espécies silvestres

Abstract

Within the Neotropical region there are 17 species of deer, nine of which occur in the national territory. These species have suffered pressures on their habitat, which causes segregation between populations, a decrease in gene exchange and genetic diversity, an increase in inbreeding and, consequently, a greater risk of extinction. The present work aimed to obtain, isolate, cultivate and cryopreservation of somatic cells obtained from feces of goats. It was observed that the vast majority of the cells analyzed were colonocytes, both isolated and aggregated, in addition to the fact that the darker the pellet was classified, the greater the amount of total and viable cells obtained, suggesting that the presence of fecal material did not affected the quality of the cells, not causing their mortality. Time had a significant negative effect on the amount of total and viable cells obtained, that is, the more time passed from the moment of defecation until the evaluation, the smaller the amount of cells obtained and viable cells. Furthermore, it was observed that half of the cells remained viable after cryopreservation by the slow method using DMSO.