Efeito da farinha de vísceras hidrolisada em alimentos extrusados para cães na digestibilidade e função renal

Abstract

Hydrolyzed poultry viscera meal (FVH) is a potentially functional ingredient that has been used in pet food. Recent works have shown that in addition to being a source of protein used in hypoallergenic foods, FVH has bioactive peptides that are able to beneficially modulate physiological mechanisms, such as regulating blood pressure through the inhibition of the angiotensin-converting enzyme (ACE). Thus, the present study aimed to: evaluate the effect of the consumption of two diets, one with conventional viscera meal and the other with the inclusion of 10% of FVH, on the renal function of healthy dogs; to evaluate the antihypertensive capacity of the diets. The experiment was carried out at two private kennels in João Pessoa and Campina Grande, both cities in Paraíba. Sixteen adult male dogs of different breeds and sizes, aged 1 to 6 years and body condition score 4 or 5 out of 9, were selected (Laflamme, 1997). Renal assessment was performed from blood samples taken on days 0, 60, and 120 by standard venipuncture using vacuum blood collection systems; Simultaneous urine samples were obtained by cystocentesis. The following analyzes were performed: creatinine, Na and K. Urinalysis included urine specific gravity, reagent strip examination, microscopic evaluation of urinary sediment, urinary creatinine, urinary protein/creatinine ratio (UP/C) and urinary electrolytes Na and K. Symmetrical dimethylarginine (SDMA) was measured to determine changes in renal function. Systolic blood pressure was measured in the forelimb in all dogs. The length of the cuff assembly was approximately 40% of the limb perimeter. All variables were verified for normal distribution by the Shapiro-Wilk test. To determine the long-term effect of diet, data were analyzed using two-way mixed model ANOVA with repeated measures of the [time] factor. Post-hoc comparisons of data were performed using a Tukey-Kramer multiple comparison test. Digestibility data were submitted to analysis of variance and F test. Statistical analyzes were performed using SigmaPlot v.12.0 software with a significance level of 5%. Thus, it was possible to conclude that there was no significant difference in digestibility when comparing the two treatments. The intake of nutrients showed a small difference between the diets. Aldosterone and ACE showed a reduction in both treatments. There was no significant effect of food intake on the renal function of the animals studied, thus, it is possible to affirm that the inclusion of 10% of hydrolyzed viscera meal in extruded food for dogs of different breeds and sizes is safe.