Avaliação hematológica e bioquímica em codornas de postura com dieta contendo superdosagem de fitase sob diferentes condições térmicas

Abstract

The objective of this study was to establish the best levels of phytase in the diet at different temperatures for laying quails (42 days) of the lineage (Couturnix japonica). Phytase has been used in poultry feed as a way to increase the availability of minerals, such as calcium and phosphorus, which are present in grain phytate indications. Temperature is another factor of great importance for the growth, development and productivity of quails, with thermal stress being an obstacle to maximizing the production of laying hens. Samples from this study were processed shortly after collection for hematological parameters and blood tissue preparation; centrifuged to obtain serum for the biochemical study. Five groups were used, with 8 animals, for each temperature, which contained a control group and four groups with different levels of phytase (0, 500, 1000, 1500 and 3000FTU/Kg). The following parameters for hematology should be measured: Hematocrits, Leukocytes, Hemoglobins, Erythrocytes, Platelets, Heterophils, Lymphocytes, Eosinophils, Basophils, Monocytes and Neutrophils, and the Heterophil:Lymphocyte (H:L) ratio. For biochemical evaluation, the following parameters were used: GGT, ALT, AST, Alkaline Phosphatase, Phosphorus, Calcium, Urea, CK and Uric Acid. Results: the addition of the enzyme phytase at levels 1500FTU and 3000FTU provided better results in relation to the blood concentration of Ca and P, contributing to the flow being maintained, thus helping the organism to remain stable. With regard to liver and kidney enzymes, there was a relevant increase in urea in the fourth laying cycle, with emphasis on the inclusion of phytase 3000FTU in the 36ºC environment, as well as an increase in alkaline phosphatase, with a higher number in the diet containing 1000FTU of phytase in the 24ºC environment. As for the calcium results, they all remained below the reference values for birds, as well as phosphorus, except for the inclusions of 1500 and 3000FTU in the 36ºC environment in the fourth cycle. Concluding that the best results were found in the environment of 30ºC with the inclusion of 1500 and 3000FTU in the diet, regarding renal and hepatic alterations, more specific studies are carried out.