ATIVIDADE ANTIMICROBIANA DE CARREADORES LIPÍDICOS NANOESTRUTURADOS DE ÓLEO ESSENCIAL DA Chamomilla recutita E CLOREXIDINA COM POTENCIAL APLICAÇÃO NO TRATAMENTO DE QUEIMADURAS

Abstract

The use of Chamomilla recutita essential oil, associated with chlorhexidine, encapsulated in manometric systems, may be a promising option for the management of burns due to its anti-inflammatory, antimicrobial and healing properties. Objective: To analyze the chemical composition of Chamomilla recutita oil and evaluate its anti-inflammatory and healing properties in silico, to incorporate these actives in nanostructured lipid carriers (NLC), to characterize the nanosystem and to evaluate its antimicrobial properties in vitro. Methods: The essential oil composition was analyzed by gas chromatography coupled to mass spectrometry (GC-MS), and the anti-inflammatory and healing activity of α-bisabolol was evaluated in silico by molecular docking. CLNs were developed and pre-formulation studies, size analysis, polydispersity index, zeta potential, fourier transform infrared spectroscopy (FTIR) and in vitro antimicrobial activity were performed. Results: α-bisabolol was the main constituent of the oil. Molecular docking demonstrated affinity prediction of α-bisabolol for the evaluated targets. The pre-formulation study allowed prediction of good structural characteristics. Particles were observed between 86.8 and 257.8 nm and zeta potentials between -12.3 to +12.7. FTIR confirmed the encapsulation of the actives and it was observed potentiated antimicrobial action of them when encapsulated. Conclusion: It was possible to develop a new system for the encapsulation of Chamomile oil and Chlorhexidine with particles within the nanometric scale and potentiated antimicrobial activity in vitro, and its use is seen as promising in the management of burns and prevention of secondary infections.