Suplementação de Lactiplantibacillus plantarum CNPC003 promove alteração na microbiota intestinal em modelo murino de depressão e ansiedade

Abstract

More than seven million Brazilians are affected by Major Depressive Disorder (MDD) and anxiety disorder, disabling pathologies that generate impacts in the social and financial spheres. The study of the intestinal microbiota showed an intrinsic relationship with the Central Nervous System (CNS) where changes in this system alter the microbiota and the opposite is also true, also impacting TDM. Thus, the present study aims to investigate the impact of the modulation of the population of Lactobacillus sp. of the gut microbiota in an animal model of MDD and anxiety, thus evaluating the gut-brain axis. The research project was approved by CEUA/UFPB (# 5467061021). Female Swiss (Mus musculus) mice (2-3 months) were used. Initially, the animals were induced to develop depressive and anxious behavior through the administration of dexamethasone DEXA (20μg/kg) and ATB solution, composed of Vancomycin (0.5 g/L), Ampicillin (1 g/L), Norflaxacin (1 g/L) and Nystatin (100.00 IU) (ad libitum). The groups were divided: Salina, Plantarum, Dexa, Dexa+PL, ATB, ATB+PL. The following behavioral tests were performed: Open field test, tail suspension test and elevated plus maze test. In addition to checking the estrous cycle phase and microbiological analysis to quantify the fecal population of Lactobacillus sp. The results show that both protocols, DEXA and ATB, managed to induce depressive-like behavior, as well as anxious-like behavior, in addition to causing a significant decrease in the fecal population of Lactobacillus sp. The administration of 200 μL of probiotic solution containing Lactiplantibacillus plantarum CNPC003, at a concentration of 109 CFU/mL, managed to reverse the depressive and anxious state, as well as the Lactobacillus sp. of the intestinal microbiota. Treatment with the probiotic solution of Lactiplantibacillus plantarum CNPC003 reversed this behavior.