Viabilização da coleta e análise seminal de serpentes mantidas em cativeiro

Abstract

The research aimed to develop an efficient method for collecting and characterizing semen from snakes kept in captivity, contributing to the conservation of endangered species. Fifteen male snakes housed in zoos in the state of Paraíba were used. Physical parameters such as color and appearance, chemical parameters such as pH, and microscopic parameters including motility, vigor, concentration, and sperm morphology were evaluated. The method demonstrated high efficacy, yielding positive results even in species with dense musculature, expanding the understanding of their reproductive peculiarities. The analyzed spermatozoa showed high quality, with progressive motility above 70% in most samples and a high percentage of normal cells. The results reinforce the importance of proper management and the application of biotechnologies in assisted reproduction and species conservation. This study provides valuable insights for improving reproductive techniques, as it details a new semen collection method for snakes that can be used in both in situ and ex situ conservation, highlighting the essential role of science in mitigating the impacts of human activities on wildlife.