Hidroxiapatitas modificadas com prata aplicadas como agentes antimicrobianos

Abstract

Hydroxyapatite has been the subject of research because of being similar to the crystal phase of the crystalline phase and the bone mineral part of the teeth, moreover possesses good biocompatibility, therefore it has become quite a biomaterial used today. In this study, hydroxyapatites have been modified with silver obtained by the method of coprecipitation and impregnation with a view to implementing the various solids such as antimicrobial agents. The synthesis started by coprecipitation of calcium chloride and dibasic ammonium phosphate using buffer such as sodium hydroxide and silver nitrate at different concentrations in amounts of 0.25, 0.5 and 1 mol% with respect to the calcium to obtain the solid doped in different percentages. In the method for impregnating the pure hydroxyapatite reacted with aqueous solutions of silver nitrate in concentrations of 0.125, 0.25 and 0.5 mmol dm -3 at room temperature. The obtained solids were characterized by X-ray diffraction, infrared spectroscopy, Raman spectroscopy and scanning electron microscopy. The results of X-ray diffraction showed the presence of metallic silver in 2Θ≈38º compounds besides the peaks corresponding to the stoichiometric hydroxyapatite. The modified hydroxyapatites with silver obtained by impregnation and co-precipitation of proved to be effective in inhibiting bacterial growth when tested in vitro bacterial adhesion through direct contact and method for Gram positive and Gram negative bacteria showing its potential for use as an antimicrobial agent.