Estudo In Vitro da creolina® sobre o veneno botrópico: Achados bioquímicos e hematológicos

Abstract

Acute ophidiasis is an important cause of morbidity and mortality in both animals and man, in Latin America, 90% of these accidents are attributed to the genus Bothrops. Although recommended, the antiofidic serum may not resolve local lesions from the proteolytic action of the Botrópico venom, in addition to its difficult acquisition. In this context it is necessary to find alternative substances with antiofidic potential. The experimental design consisted of five groups, each containing five samples, G1 - control group, G2 - blood + venom, G3 - blood + Creolina ®, G4 - blood, venom and Creolina ®, G5 - blood + venom / Creolina ®. Each sample contained 2ml of bovine blood, packed in eppendorf (2.5ml) with 2μl of EDTA. Blood was collected from calves up to one year old, without racial or sexual distinction, from the Federal University of Paraíba. Each sample was established from randomly selected. Groups G2, G4 and G5 received 2μl of botrópico venom and groups G3, G4 and G5 received 1μl of Creolina®, values predetermined by the research team. This step was performed after individual evaluation of the samples. The results were evaluated through ANOVA and Newman-Keuls, at 5% significance. The samples that presented significance in the statistical method were only those of group G3, showing a hemolytic capacity and increase of the fibrinogen. These results show that botulent venom in the volume of 2μl did not trigger hematological changes in vitro, and that the use of Creolina® in the volume of 1μl did not demonstrate antiofidic activity, but caused hemolytic disorders in the other samples in which it was present.