Detecção e caracterização molecular de enteropatógenos em caprinos do Estado da Paraíba

Abstract

The economic losses determined by neonatal diarrhea to the economic exploitation of animals leads both directly and indirectly to an increase in the costs of breeding. Due to the lack of studies, the etiology of neonatal diarrhea in caprine herds of the northeast region of Brazil is systematically and generally conferred to helminthes. The importance of bacteria, protozoa and mainly viruses in the etiology of the episodes of neonatal diarrhea in caprine and ovine cattle is not yet properly characterized. The aim of this project was to evaluate the presence of enteropathogens involved in occurrences of diarrhea in herds of caprine in the state of Paraíba. The methodology consisted of visits to the caprine herds of mesoregions of the state of Paraíba. Was collected two hundred twenty diarrheal stool samples from caprine up to 90 days of age. The samples were submitted to rotavirus identification by ss-PAGE. The isolated strains were submitted to biochemical tests to confirm bacterial species, followed by the determination of serogroups and genotypic groups, as well the phylogenetic analyses. The samples were also submitted to antimicrobial susceptibility testing. Each one of the samples were negative to rotavirus. Isolates were identified to E. coli, S. typhi, Shigella sonnei and Enterobacter classified phylogenetically in (9/39), D (19/39), B1 (7/39) and A (4/29). The virulence factors founded were K99 (04/39) and Stx (02/39). There were no positives isolates for K88, Sta,StbP and LT. To the antimicrobial susceptibility testing, the isolates were sensitive to Chloramphenicol, streptomycin, Amoxicillin and Ciprofloxacin, all of them being resistant to Lincomycin, Vancomycin and penicillin. The results showed that the samples derived from caprine from the state of Paraíba showed E. coli belonging to phylogenetic group B2 (9-23,07%) and D (19 –48,71%), and a high incidence of resistance against tested antibiotics.