Análise comparativa da microbiota oral e cloacal de serpentes peçonhentas e não peçonhentas de um criadouro de répteis no estado da Paraíba.

Abstract

This study aimed to analyze the isolated microbiota of cloacal cavity (cavum cloacarum) and oral cavity (cavum oris) of venomous and non-venomous snakes from a reptile zoo in the state of Paraíba - Brazil. For this purpose, 20 snakes were used, 10 venomous specimens, from the family Viperidae, two of them Bothrops neuwiedi, three Bothrops erythromelas, two Bothrops leucurus, one Bothrops moojeni and two Crotalus durissus and 10 non-venomous specimens belonging to the Boidae family, all of them Boa constrictor species. All the samples were

plated on 4% horse blood agar, MacConkey agar and the XLD agar (Xylose-Lysine- Deoxycholate). The obtained bacterial genera were: Shigella spp. (20,41%), Yersinia spp.

(18,37%), Salmonella spp. (14,29%), Edwardsiella spp. (10,20%), Citrobacter spp. (8,16%), Providencia spp. (8,16%), Arizona spp. (6,12%), Enterobacter spp. (6,12%), Klebsiella spp. (2,04%), in addition to Non-fermenting Gram-negative Bacilli (NFGNB) (6.12%). The genera Providencia spp, Arizona spp, Klebsiella spp. and the NFGNB were only shown in samples from venomous snakes, while the genera Edwardsiella spp. and Enterobacter spp. were only shown in samples from non-venomous snakes. The only genus found in the oral and cloacal cavity of two studied groups was the genus Yersinia spp. Colonies isolated from cloacal samples represent 73.46% of all colonies isolated in this study against 26.54% from oral samples. Colonies from venomous snakes represent 45.90% of all colonies isolated in this study, against 55.10% from non-venomous snakes.