Obtenção da enzima xilose redutase a partir do bagaço de abacaxi (Ananas Comosus L. Merril)

Abstract

Pineapple (Ananas Comosus L. Merril) has a great influence on the national market due to its high demand. This factor makes Brazil one of the main world producers of this fruit. Due to its high industrial activity, a large amount of bagasse is generated. The constituents of bagasse are rich in lignin, cellulose and hemicellulose. The hemicellulose content is a highlight of this biomass. Formed mainly by xylans, the acid hydrolysis of this material allows an optimal conversion of this residue into xylose-rich liquor. A strong application of this obtained xylose is its conversion into xylitol. The conversion takes place mainly by the action of the enzyme xylose reductase with the participation of the coenzymes NADPH or NADH, present in the metabolism of yeasts. Among the yeasts identified as producing xylose reductase, those of the genus Candida stand out for their greater conversion efficiency. Therefore, the present work aims to use bibliographic research in decision making to study the cell disruption process adopted in order to promote the extraction of the enzyme xylose reductase (XR), using Candida Tropicalis as the process yeast. This study is of great importance, since obtaining XR from pineapple bagasse is a topic little explored in the scientific community and can generate great gains in industrial processes.