Sequenciamento, montagem e anotação do genoma de uma cepa Salmonella Derby ST13 originada de avicultura de postura no Brasil

Abstract

The bacteria Salmonella Derby can cause non-typhoid salmonellosis in humans and animals. Swine are their main reservoirs but they can also be found in birds.The genomic characterization of different strains of Salmonella serovarsis is important to understand the contamination sources and transmission routes in anial production systems, allowing the implementation of appropriate control measures. The present study aimed to characterize the genome of Salmonella Derby strain SD115 originated from laying poultry in Brazil. Whole genome sequencing (WGS) was performed on the Illumina MiSeq platform. Genome assembly and annotation were performed by means of the Unicycler version 1.0 program PathoSystems Resource Integration Center (PATRIC) server, respectively Multilocus sequence typing was determined by the MLST 2.0 software. Antimicrobial resistance genes and virulence factors were identified by Comprehensive Antibiotic Resistance Database (CARD) and Virulence Factor of Pathogenic Bacteria Database (VFDB), respectively. The presence of plasmids was investigated by PlasmidFinder. The S. Derby strain SD1112.04 was identified as sequence type 13 (ST13) and its genome had a total size of 4,787,671 pb, with 52.04% C+G contents and 4,820 coding sequences (CDSs),from which 650 were associated with hypothetical proteins. We identified 99 virulence factors and 49 antimicrobial resistance genes against eight different classes of antibiotics. The results of the present staudy can be used to support epidemiological and evolutionary research on S. Derby