Produção de compostos bioativos em biorreator utilizando resíduo agroindustrial

Abstract

Nutraceuticals are bioactive molecules that can be used to meet the nutritional needs of the body, while helping with health as a whole. Many efforts have been used to develop technologies that optimize the production of these biomolecules by microorganisms, and in this sense, several methodologies are intelligently employed, such as the use of integrated bioreactors. Currently, special attention has been given to biotechnological processes as an alternative to chemical routes due to low production costs. The use of residues is quite convenient both from the point of view of sustainability and economics when they are used as a substrate for the development of microorganisms and, consequently, in the production of a compound of high added value such as biomolecules. The objective of this work was to study the production of carotenoids, lipids and biomass in an integrated Bioreactor using the yeasts Rhodothorula glutinis and Paffia rhodozyma as producing agents and to evaluate the feasibility of using cassava as a substrate for this production. Biomass, lipids and carotenoids produced were analyzed. The physicochemical analyzes of the manipueira were carried out and found 93.54% of moisture, 32.24 g/L of reducing sugars, 47.18 g/L of total sugars, 1.58% of proteins, 1% of ash, 7.3 °Brix of total soluble solids and pH of 5.3. The best results found during individual yeast cultures in bioreactor: 8.57 g/L and 9.85 g/L of biomass, 0.81 g/L and 4.19 g/L of lipids and 1.3 mg/L and 1.1 mg/L of total carotenoids for R. glutinis and P. rhodozyma, respectively. Whereas during the mixed culture, 7.15 g/L of biomass and the highest amounts of lipids (6.04 g/L) and total carotenoids (1.51 mg/L) were produced when compared to the individual cultures. The microorganisms used in this study showed significant growth when cultivated in a bioreactor in a medium containing manipueira as a carbon source and, therefore, these results support the feasibility of using manipueira as a substrate to be used in a biotechnological process with the objective of producing carotenoids and lipids and microbial biomass.