Modelagem e simulação de um biorreator CSTR para produção enzimática de oligossacarídeo panose

Abstract

Panose is a trisaccharide consisting of a maltose molecule linked to a glucose molecule by an α-1,6-glycoside bond. This trisaccharide has the potential to be used in several areas such as: food, beverages, pharmaceuticals and medicines due to its characteristics as a non-cariogenic sweetener, since the oral flora does not ferment it, it is also considered prebiotic for stimulating the growth of microorganisms, such as lactobacilli and bidifidobacteria, and by inhibiting the growth of unwanted microorganisms such as E.coli and Samonella. Despite its potential applications, this carbohydrate is not produced on a large scale and there are still few studies and research. Allied to the characteristics of the bioreactor, mathematical modeling emerges as a powerful tool for the study and understanding of the behavior of enzymatic processes, allowing the evaluation of changes, such as changes in operational parameters, aiming at optimizing systems. In this sense, this work aimed to develop a mathematical model of a CSTR bioreactor associated with the bypass model and dead zone for enzymatic production with dextran-sucrase through Leuconostic mesenteroídes NRRL B-512F and simulation using the computational software Scilab as a tool. The results obtained showed that for a better production of panose it is necessary to maintain the initial maltose concentration of at least 400 mmol/L and the simulation of the proposed model resulted in an enzymatic kinetics with satisfactory data, because through the model it was possible to obtain at the end of the process a panose concentration of 128.36 mmol/L (Q0=0.025m3/s) and 121.07mmol/L (Q0=0.05m3/s) obtaining the highest conversion of 0.878%. With the kinetic data, the CSTR reactor was modeled and the impact that the different dilution rates had on the process simulation was compared, where the results show that the effects of non-ideality affected the production of dextran and panose, in terms of both α and β parameters and initial maltose concentrations.