Aplicação de células de Geotrichum candidum na Reação de Baeyer-Villiger em Acetofenona

Abstract

Biocatalytic processes are extremely important in obtaining low-cost chemical products for high value-added processes. In addition, most biotransformation processes are carried out using biodegradable materials under normal temperature and pressure conditions, maintaining good reaction performance compared to traditional synthetic methods due to their high specificity and chemical selectivity. This work aims to study the characteristics of the fungus Geotrichum candidum CCT 1205 in the Baeyer-Villiger oxidation reaction of acetophenone, controlling variables such as the volume of substrate and quantity of biomass. In the oxidation reaction of 20 μL of acetophenone substrate, 95.8% conversion of acetophenone to phenol was confirmed by hydrolysis in 2 g of wet biological material and 81.2% with 3 g after 36 hours of reaction. The effect of the volume of substrate was also significant, with 89% of phenol converted in just 24 hours when 20 μL of acetophenone was added to 2 g of biomass, making it the ideal amount of substrate to add. The quantity of biomass and substrate responses were monitored by GC-FID. It is through these studies that it is possible to understand the properties of Geotrichum candidum, such as its optimum conditions in reaction with acetophenones for application in future work with other types of substrates.