Montagem de um sistema de microscopia holográfica digital

Abstract

Digital Holographic Microscopy (DHM) is a powerful interferometric technique that enables high-resolution, label-free, three-dimensional reconstruction of microscopic phase objects. This project aims to develop a robust DHM system based on a Mach-Zehnder interferometer architecture, precisely aligned on an inertial table to ensure phase stability. The system is designed for the acquisition and numerical reconstruction of digital holograms, providing quantitative phase contrast imaging. We present a comprehensive theoretical and practical framework for the system’s assembly, alongside a detailed analysis of the numerical methods employed for holographic reconstruction. The capabilities of our apparatus are demonstrated through the imaging of human red blood cells, highlighting its potential for medical diagnostics—particularly in identifying morphological abnormalities associated with conditions such as sickle cell anemia. Our results underscore the viability of DHM as a powerful tool for non-invasive biological analysis and pave the way for future integration with automated disease screening protocols.