Fermentação alcoólica do hidrolisado de manipueira.

Abstract

The establishment of agreements between the different countries of the world, with the aim of reducing the emission of greenhouse gases, has aroused the interest and development of research, in the sense of producing and using renewable energy sources. Among these are wind, solar and biomass energies. Brazil is a reference country in the production of ethanol, from the sugarcane juice. However, in recent years, there has been a worldwide tendency to increase ethanol production, also using agro-industrial waste as raw materials, since some of these are discarded in the environment, without any previous treatment, acting as agents Polluters Among these residues, in the case of Brazil, there is the manipueira, liquid obtained from the pressing of manioc (Manihot esculenta Crantz), in its processing for the production of flour. The manipueira has a milky appearance, light yellow color and strong odor and is highly polluting. In order to add value to the residue, an analysis was made on the potential of ethanol production. The work was carried out in two stages. In the first, the manipulative residue was enzymatically saccharified under the following conditions: alpha-amylase enzyme (1 mL enzyme / 100 mL substrate), 75 °C, pH 5.0, for 54 hours. The second stage was related to the alcoholic fermentation of the hydrolyzate obtained in the first stage. The fermentative agent used was Sacccharomyces cerevisiae yeast (UFLA CA-11). The total volume of must was 150 mL, the fermentation being conducted in a shaker incubator (LUCA-223) at 100 rpm, 30 °C and pH 6.0. The process was monitored by the reduction of soluble solids content (ºBrix), and was considered finished when ºBrix remained constant after 4 consecutive readings. With this, the process was finalized between 26 and 27 hours of fermentation. At the end of the process there was a significant consumption of the reducing sugars, in the average of 93%. The average yield of ethanol relative to the theoretical was 65.59±1.83(%).