Viabilidade de produção lipídica da microalga coelastrum cambricum utilizando meio de cultura alternativo e estresse nutricional

Abstract

A large proportion of the population of developing countries with nutritional deficiencies, mainly in difficulty of access to diets rich in healthy lipids. Studies on microalgae seek the nutritional utilization of several species, mainly for the extraction of grease to be used as an ingredient in food. The objective of this work is to analyze the viability of biomass and coagulation production through optimization of the culture with the alternative culture medium (Biocomposto Hortifrúti) and nutritional stress with nitrogen deprivation. Coelastrum cambricum, selected from the Culture Bank of the Laboratory of Environments Recife and Biotechnology with Microalgae (LARBIM / UFPB). Nature was isolated from the Riacho da Bica located in Parque Arruda Câmara of the city of João Pessoa-PB. UF for cultivation in the control medium and 232.66 UF in the alternative medium for seven days 299.0 UF for the cultivation in the control medium and 465.0 UF for the cultivation. In alternative medium with stress application, which also presented higher of Growth (k). The maximum cell yield was 9.25 cells.L-1x105 and 14.4 cells.L-1x105 in alternative medium without and with nutritional stress, respectively. Lipid values were higher for cultures with nutritional stress. The lipid contents found in the gravimetric methodologies were higher in relation to the spectrophotometric analysis. It is concluded that an application of alternative culture media for synthetic media and nutritional stress with nitrogen deprivation are satisfactory for the cellular physiological and biochemical development of C. cambricum, besides optimizing the yield in microalgal biomass of this species. The lipid yield of the species under normal conditions of cultivation is small, however, the increase in lipid contents was approved by the application of culture media and nutritional stress with nitrogen deprivation. Thus, two promising methods for a reduction of costs and production of microalgae biomass and grease matter through chlorophyll C. cambricum.